Identification of mutations in SARS-CoV-2 PCR primer regionsArticle Published on 2022-11-042022-11-15 Journal: Scientific Reports [Category] SARS, 변종, 치료기술, 치료제, [키워드] Accuracy Analysis anticipated approach dataset diagnostic Efficiency genomic variation gold standard identification Mutation Neutral omicron overlapping PCR PCR efficiency primer primer set primer sets Region RT-PCR Sample SARS-CoV-2 SARS-CoV-2 PCR SARS-CoV-2 viral strain susceptible target region the SARS-CoV-2 genome variant Variation [DOI] 10.1038/s41598-022-21953-3 PMC 바로가기
Evidence of co-infections during Delta and Omicron SARS-CoV-2 variants co-circulation through prospective screening and sequencingArticle Published on 2022-11-012022-11-15 Journal: Clinical microbiology and infection : the official [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] analysis chimeric circulation Co-infection collected COVID-19 Delta Delta and Omicron VOC Epidemic epidemiologically evidence of France generate Genetic Health Infection K417N L452R Mutation nasopharyngeal sample omicron Pandemics pathogenic patients PCR assay polymorphism primer sets profile Recombination recombination event Respiratory samples S-gene mutation Sample SARS-CoV-2 SARS-CoV-2 co-infection SARS-CoV-2 genome SARS-CoV-2 variant screened sequence Sequencing single nucleotide Single nucleotide polymorphism Single nucleotide polymorphism screening SNP SNPs submitted Surveillance variant virus VoC was performed WGS whole genome Whole genome sequencing whole genome sequencing. [DOI] 10.1016/j.cmi.2022.06.030 PMC 바로가기
Quantification of SARS-CoV-2 RNA in wastewater treatment plants mirrors the pandemic trend in Hong KongArticle Published on 2022-10-202022-11-15 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 치료기술, [키워드] applicability average Characteristics collected Community Concentration conducted contributing to correlation ENhance Epidemiology evaluated fecal Frequency Hong Kong key factor longitudinal Longitudinal monitoring MONITOR pandemic pathogen plant primer sets provide public health RNA SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 RNA SARS-CoV-2 virus supernatant Surveillance the SARS-CoV-2 virus Treatment ultracentrifugation virus was normalized wastewater WBE WWTP. [DOI] 10.1016/j.scitotenv.2022.157121 PMC 바로가기
Wastewater-based epidemiology in countries with poor wastewater treatment – Epidemiological indicator function of SARS-CoV-2 RNA in surface waters폐수 처리가 열악한 국가의 폐수 기반 역학 - 지표수에서 SARS-CoV-2 RNA의 역학 지표 기능Article Published on 2022-10-152022-09-11 Journal: The Science of the total environment [Category] COVID19(2023년), MERS, SARS, 진단, 치료기술, 치료법, 치료제, [키워드] adaptation adoption affected alternative analysed applied approach Bacteria Belgrade collected complex complex systems Concentration country COVID-19 Danube effort epidemiological epidemiological purposes Epidemiology Factor Factors Faecal pollution Genes Genetic human populations identify indicated methodology N1 and N2 nucleocapsid outbreak parameter physicochemical primer set primer sets Protein qualitative data quantification quantified Quantitative recipient river RNA RT-qPCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 surveillance Sava Serbia surface Surface waters Surveillance Treatment Untreated wastewaters. Volume water [DOI] 10.1016/j.scitotenv.2022.156964 PMC 바로가기 [Article Type] Article
Identification of conserved regions from 230,163 SARS-CoV-2 genomes and their use in diagnostic PCR primer design230,163 SARS-CoV-2 게놈에서 보존 영역 식별 및 진단 PCR 프라이머 디자인에서의 사용Article Published on 2022-08-012022-09-11 Journal: Genes & genomics [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] addition Alpha amplification approach Beta bioinformatics can be used candidate Candidates characteristic conserved Coverage dataset Delta Diagnosis diagnostic Epsilon Eta evaluated exhibited False negative full-length Gamma genome sequence genomes genomic sequence GISAID GISAID EpiCoV database identification identify in silico Intensive Iota Multiple Multiple sequence alignment multiple sequence alignments NCBI nine NSP2 orf3a gene Outbreaks pandemic PCR PCR primer primer primer set primer sets Primers qPCR RdRP Region removal retrieved RNA sample RNA samples RT-qPCR S gene SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genomes SARS-CoV-2 variant SARS-CoV-2. sequence Sequence alignment target viral genome viral sequences was reduced Zeta [DOI] 10.1007/s13258-022-01264-7 PMC 바로가기 [Article Type] Article
Improving RT-LAMP detection of SARS-CoV-2 RNA through primer set selection and combination프라이머 세트 선택 및 조합을 통한 SARS-CoV-2 RNA의 RT-LAMP 검출 개선Article Published on 2022-04-012022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] adoption approach assays breadth Combination conditions Detection sensitivity Diagnostic method Effect evaluate expand identify Improving isothermal amplification knowledge LAMP Loop-mediated isothermal amplification molecular nucleic acid nucleic acid testing observé primer primer set primer sets prominence RT-LAMP RT-qPCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2 RNA sensitivity Surveillance targets Transcription utility while [DOI] 10.1371/journal.pone.0254324 PMC 바로가기 [Article Type] Article
Profiling RT-LAMP tolerance of sequence variation for SARS-CoV-2 RNA detectionSARS-CoV-2 RNA 검출을 위한 서열 변이의 RT-LAMP 내성 프로파일링Article Published on 2022-03-242022-09-11 Journal: PLoS ONE [Category] SARS, 변종, 치료기술, 치료제, [키워드] adopted affecting amplification amplify bases clinical laboratory creating Critical deletions Detection sensitivity diagnostic diagnostic test diagnostic tests dramatic increase Effect highlight identify knowledge Laboratory LAMP molecular molecular diagnostic tests Mutation no effect nucleic acid nucleic acid amplification Point mutation primer primer set primer sets Profiling qPCR assays Reactions reduced regions RNA sequence robust RT-LAMP RT-qPCR RT-qPCR assays SARS-CoV-2 SARS-CoV-2 pandemic SARS-CoV-2 RNA detection SARS-CoV-2 sequence SARS-CoV-2 variant SARS-CoV-2 variants sensitivity sequence variation significantly Spread These data Tolerance utility variant Viral RNA viral RNA detection virus [DOI] 10.1371/journal.pone.0259610 PMC 바로가기 [Article Type] Article
High-Integrity Sequencing of Spike Gene for SARS-CoV-2 Variant DeterminationSARS-CoV-2 변이체 결정을 위한 스파이크 유전자의 높은 무결성 시퀀싱Article Published on 2022-03-172022-09-11 Journal: International Journal of Molecular Sciences [Category] SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] amplicons approach ARTIC Network consensus sequence Coverage datasets demonstrated detect detection rate Determination determine Disadvantages evaluated Express FASTQ in silico mutations Nanopore nanopore sequencing overcome Oxford PCR PCR primer PCR primers primer primer set primer sets Primers protocol provided Region robustness SARS-CoV-2 SARS-CoV-2 genome sequence sequenced sample sequenced samples Sequencing spike spike gene Spike protein the SARS-CoV-2 genome variant Variant. variants [DOI] 10.3390/ijms23063257 PMC 바로가기 [Article Type] Article
Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCRTaqman 기반 및 SYBR 녹색 기반 RT-QPCR에 의한 SARS-COV-2 프라이머 세트의 분석 감도 및 효율 비교Methods and Protocols Published on 2022-02-262022-09-01 Journal: Applied Microbiology and Biotechnology [Category] COVID19(2023년), Fulltext, MERS, SARS, 변종, 진단, 치료기술, [키워드] alternative method analytical sensitivity approach best Combination comparable coronavirus disease Coronavirus disease 2019 country COVID-19 Delta detect Diagnosis diagnostics Efficiency Eta evaluated gold gold standard Health Organization Lambda molecular omicron ORF1 ORF1ab pandemic Patient Prevent primer set primer sets public health RT-qPCR SARS-CoV-2 SARS-CoV-2 detection sensitivity specificity supplementary material SYBR Green TaqMan TaqMan probe Vaccine variant variants WHO World Health Organization [DOI] 10.1007/s00253-022-11822-4 PMC 바로가기 [Article Type] Methods and Protocols
Accuracy of Real-Time Polymerase Chain Reaction in COVID-19 PatientsCOVID-19 환자의 실시간 중합효소 연쇄 반응의 정확도Research Article Published on 2022-02-162022-09-10 Journal: Microbiology Spectrum [Category] COVID19(2023년), MERS, SARS, 치료기술, 치료법, 치료제, [키워드] E gene NP gene RdRp-gene Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addressed Analysis assays caused CDC CDC N1, N2 center chain Characteristics Clinical practice clinical samples conducted Control coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 COVID-19 patient cutoff value cycle threshold demonstrated Diagnosis diagnostic Diagnostic accuracy diagnostic specificity discrepancy disease control enrolled followed by Health Organization highest house lowest majority Mild nasopharyngeal nasopharyngeal sample Nasopharyngeal samples nucleocapsid protein Numerous Oropharyngeal oropharyngeal samples pandemic prevention primer primer set primer sets Primers Prospective Study qPCR qPCR analysis RdRP reaction Real-time PCR real-time polymerase chain reaction Research respiratory Respiratory illness RT-PCR RT-qPCR RT-qPCRs Saliva SARS-CoV-2 SARS-CoV2 sensitivity Sensitivity and specificity Seroconversion severe acute respiratory syndrome Coronavirus severe respiratory illness significantly specificity specimen specimens sputum Sputum sample Viral Viral detection WHO World Health Organization [DOI] 10.1128/spectrum.00591-21 PMC 바로가기 [Article Type] Research Article